In Vivo Imaging of Beta-cell Receptors by Applied Nano Technology

Karolinska Institutet (KI), Stockholm, Sweden

Working Group Leader

Professor Per-Olof Berggren

Department of Molecular Medicine and Surgery
Karolinska Institutet
Karolinska University Hospital Solna, L1:03
171 76 Stockholm
Phone: +46 8-517 757 31
Fax: +46 8-517 794 50
E-mail adress: per-olof.berggren@ki.se

Group Members

Ingo Leibiger
Barbara Leibiger
Helena Nässén
Britt-Marie Witasp

Principal scientific and technical personnel
Team leader: Professor Per-Olof Berggren is Professor and Director of The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet. Visiting research Professor, Mary Lou Held Professor of Diabetes Research, at the Diabetes Research Institute, University of Miami, Miller School of Medicine, Miami, FL. Published 310 articles and a large number of abstracts in the field of cell physiology, signal-transduction and experimental diabetes research. His research is funded by NIH, JDRF, EFSD, EU, The Swedish Research Council, The Swedish Diabetes Association, The Novo Nordisk Foundation and a number of private foundations.
Other personnel:
Ingo Leibiger, MD/PhD, Associate Professor (functional β-cell mass, fluorescent constructs, signal- transduction)
Barbara Leibiger, MD/PhD, Associate Professor (functional β-cell mass, fluorescent constructs, signal- transduction)

Expertise. Principal investigator: The research conducted by in the laboratory of Per-Olof Berggren is focused on the molecular mechanisms underlying signal-transduction in the insulin-secreting pancreatic under normal conditions and why these do not function properly in diabetes. He has been in the field for more than 20 years.
Research: The specific interests are on the dynamics in glucose metabolism, the phospholipase C system, autocrine and paracrine feedback loops, insulin receptor signaling, regulation of cytoplasmic free Ca2+-concentration, gene transcription, proteins regulating exocytosis, and the role of the voltage-gated Ca2+ -channels in β-cell both physiology and pathophysiology. Using specific methodologies, the first steps have recently been taken towards evaluation of β-cell signal-transduction under in vivo conditions and in situ.
Skills: Pancreatic β-cell signal-transduction under normal conditions and in diabetes.
Responsibilities: Member of the Medical Science Review Committee of the Juvenile Diabetes Foundation International, New York, 1992-2001. Member of the Science Review Committee for the Swedish Diabetes Association 1998-2002. Member of the Metabolism Study Section, NIH, 2001. Member of the Nobel Assembly at Karolinska Institutet since 1999. Member Editorial Board of Diabetes 2000-2002. Member Editorial Board of American Journal of Physiology, Endocrinology and Metabolismm 2001-2007. Member Editorial Board Cell Metabolism since 2005.
Participation in EU programmes: BIO-98-0286: developments of genetically engineered highly competent insulin-secreting cells (1998-2000), TONECA 2004, EuroDia FP6-518153: Functional genomics of pancreatic β-cells and tissues involved in control of the endocrine pancreas for prevention and treatment of type 2 diabetes (2006-2010).  

Key recent publications:

  • Kohler M, Zaitsev SV, Zaitseva II, Leibiger B, Leibiger IB, Turunen M, et al. On-line monitoring of apoptosis in insulin-secreting cells. Diabetes 52, 2943-2950 (2003)
  • Berggren PO, Yang SN, Murakami M, Efanov AM, et al. Removal of Ca2+ channel b3 subunit enhances Ca2+ oscillation frequency and insulin exocytosis. Cell 119, 273-284, (2004)
  • Nyqvist D, Kohler M, Wahlsted H, Berggren PO. Donor islet endothelial cells participate in formation of functional vessels within pancreatic islet grafts. Diabetes 54, 2287-2293 (2005)
  • Yang SN, Berggren PO. The role of voltage-gated calcium channels in pancreatic beta-cell physiology and pathophysiology. Endocrine Rev 27, 621-676 (2006)
  • Cabrera O, Berman DM, Kenyon NS, Ricordi C, Berggren PO, Caicedo A. The unique cytoarchitecture of human pancreatic islets has implications for islet cell function. PNAS 103, 2334-2339 (2006)
  • Uhles S, Moede T, Leibiger B, Berggren PO, Leibiger IB. Selective gene activation by spatial segregation of insulin receptor B signalling. FASEB J 21, 1609-1621, (2007)